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2 edition of Studies on the cultivation of vaccinia on the chorio-allantoic membranes of chick embryos found in the catalog.

Studies on the cultivation of vaccinia on the chorio-allantoic membranes of chick embryos

Great Britain. Ministry of Health

Studies on the cultivation of vaccinia on the chorio-allantoic membranes of chick embryos

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Published by H.M.S.O in London .
Written in English


Edition Notes

Statementby W.D.H. Stevenson and G.G. Butler.
SeriesReports on public health and medical subjects -- no.87
ContributionsStevenson, William David Henderson., Butler, George Guy.
ID Numbers
Open LibraryOL14391778M


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Studies on the cultivation of vaccinia on the chorio-allantoic membranes of chick embryos by Great Britain. Ministry of Health Download PDF EPUB FB2

Studies on the Cultivation of Vaccinia on the Chorio-Allantoie Membranes of Chick Embryos. During the past five years or so the authors have been intent on the possible production of chick membrane vaccinia virus on a large : W.

Stevenson, G. Butler. Goodpasture EW, Woodruff AM, Buddingh GJ. Vaccinal Infection of the Chorio-Allantoic Membrane of the Chick Embryo.

Am J Pathol. May; 8 (3)– [PMC free article] Woodruff AM, Goodpasture EW. The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection with the Fowl-Pox Virus. Am J Pathol. May; 7 (3) Cited by: Goodpasture EW, Woodruff AM, Buddingh GJ.

THE CULTIVATION OF VACCINE AND OTHER VIRUSES IN THE CHORIOALLANTOIC MEMBRANE OF CHICK EMBRYOS. Science. Oct 9;74 ()– Muckenfuss RS, McCordock HA, Harter JS. A Study of Vaccine Virus Pneumonia in Rabbits. Am J Pathol. Jan;8 (1)–Cited by: Studies on a Dermal Strain of Vaccinia Virus adapted to the Chorio-Allantoic Membrane of the Chick Embryo.

Author(s): Hassan, I. : I. Hassan. ANDERSON SG. The growth curve of vaccinia virus on the chorioallantois. Aust J Exp Biol Med Sci. Oct; 32 (5)– [BANG FB. Cellular changes in the chick chorio-allantoic membrane infected with herpes simplex and vaccinia; a study Cited by: 9.

STUDIES ON THE CULTIVATION OF THE VIRUS OF VACCINIA.* f Edna Steinhardt, C. Israeli, and R. Lambert. (From the Research Laboratory, Board of Health, and the Pathological Laboratory, College of Physicians and Surgeons, New York City.) Many investigators have shown that the virus of vaccinia.

The cultivation of vaccine and other viruses in the chorio-allantoic membrane of chick embryos. The germicidal action of the hydrogen ion and of the lower fatty acids.

The influence of inorganic salts upon : John M. Hale. By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens.

The cultures could be used for Cited by: The Susceptibility of the Chorio-Allantoic Membrane of Chick Embryos to Infection with the Fowl-Pox Virus * Alice Miles Woodruff and Ernest W. Goodpasture * Cited by: In most of the methods hitherto used for the cultivation of vaccinia virus a pure dermal strain, has not been used as an initial inoculum on account of the difficulty of ensuring freedom from contaminating bacteria.

The authors obtained bacteria-free lymph by injecting ordinary glycerinated calf lymph intradermally into rabbits. Four days later the rabbit was killed and the Cited by: The chorioallantoic membrane — also called the chorioallantois or abbreviated to CAM — is a vascular membrane found in eggs of some amniotes, such as birds and is formed by the fusion of the mesodermal layers of two developmental structures: the allantois and the chorion.

In mammals, this structure forms the placenta. Three different layers compose the chorioallantoic membrane MeSH: D The author summarizes and discusses the work done by himself and others on the cultivation of vaccine virus in chick embryo and the use of such culture virus for human vaccination.

Continuous embryo culture through generations has not altered the characteristics of the virus and intervening mammalian passage does not seem to be necessary to maintain its Cited by: 3.

Dried smallpox vaccine prepared from infected chorio-allantoic membrane extracts dried in 5% peptone [COLLIER, this Bulletin,v. 30, ] was shown to be stable when stored at 36°C. for periods, up to a year.

Experiments with bottle instead of ampoule drying were unsuccessful. However, dried vaccine sealed in " superdry " N2 at atmospheric-pressure was as stable as Cited by: 5. The most im- portant types of eulture virus which have been used are (1) the tissue- culture virus of Rivers and Ward ~-3 and (2) the vaccine virus grown on the chorioallantoic membrane of the developing chick embryo by Good- pasture, Buddingh, and co-workers~ The continued cultivation of vaecinia virus in a medium consisting of minced chick Cited by: 4.

Thc subjects covered in riffs comprehensive list include the technique of inoculation and egg management, thc focal lesions on the chorio-allantoic membranes, the cultivation of individual viruses, the cultivation of bacteria, protozoa and forcign tissues in chick-embiTos and the cultivation of normal and neoplastic tissues on the : J.

Cuthbert, G.G. Davidson. VIROL () Studies with Poxviruses by an Improved Plaque Technique J. POBTEBFIELD AND A. ALLISO N National Institute for Medical Research, Mill Hill, London, N. 7 Accepted November 9, With chick embryo cell monolayers and an agar overlay containing tris (hydroxymethyl)aminomethane, well-defined plaques were obtained with vaccinia Cited by: The chick embryo chorioallantoic membrane (CAM) assay facilitates the testing of multiple samples and the generation of dose–dilution curves, and has been used to identify almost all of the known angiogenic factors.

The allantois is an extraembryonic membrane, derived from the mesoderm in which primitive blood vessels begin to take shape on day 3 of incubation. In this study the conventional chick chorio­allantoic membrane assay was compared with a modifieation of this technique in a series of batehes using a total of ]00 eggs.

Negative and false positive results in the chick chorioallantoic membrane assay using the original and modified methods in different batches Batch Original method Modified Cited by: 9.

Cowles PB. The Germicidal Action of the Hydrogen Ion and of the Lower Fatty Acids. Yale J Biol Med. May; 13 (5)– [PMC free article] Goodpasture EW, Woodruff AM, Buddingh GJ.

THE CULTIVATION OF VACCINE AND OTHER VIRUSES IN THE CHORIOALLANTOIC MEMBRANE OF CHICK EMBRYOS. Science. Oct 9; 74 ()– Rivers TM, Cited by: 2. Chick chorioallantoic membrane (CAM) invasion by ovarian cancer cells in day 14 chick embryos. Data generated from 48–60 images from 6 chicken embryos per cell line.

Data represents the percentage of images with invasion into the mesoderm, mean ± SEM from two independent by:   The method introduced by Hoyle 1,2 in his study on the multiplication of influenza virus in egg membranes, however, did not yield clear-cut results with mumps virus.

The main difficulties were the. onto the chorio-allantoic membrane (CAM) of day-old chick embryos. Whitish "pearl" growths appeared on the CAM after several days incubation, and he was able then to pass this agent serially in eggs with material filtered through a Berkefeld V candle.

An eleventh. Stocks of Lister vaccinia virus were obtained by growing vaccine strain of Lister vaccinia on the chorioallantoic membranes of 9-dayold embryonated hens'.

The chorio-allantoic membrane of the chick embryo has been manipulated in a variety of ways in order that it might be used to study virus growth (Bever-idge and Burnet, ). The most fre-quently employed procedure consists of dropping the chorio-allantoic mem-brane from the shell membrane by ap-plying suction through a hole drilled in.

Orthopoxviruses Pathogenic for Humans covers those viruses capable of causing disease in man, including monkeypox, smallpox, cowpox, and vaccinia.

The coverage of each virus is comprehensive, covering the biology, molecular biology, and ecology of the virus as well as the clinical and epidemiological aspects of these viruses in humans and animals.

Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately kbp in length, which encodes approximately dimensions of the virion are roughly × × nm, with a mass of approximately 5–10 : incertae sedis.

Guided bone regeneration is a common dental implant treatment where a barrier membrane (BM) is used between epithelial tissue and bone or bone graft to prevent the invasion of the fast-proliferating epithelial cells into the defect site to be able to preserve a space for infiltration of slower-growing bone cells into the periodontal defect site.

In this study, a bilayer Cited by: 2. Process for preparing a virus disease live vaccine by employing culture of a quail embryonated egg or tissue culture of a quail embryo fibroblast for cultivation of the virus.

The vaccine obtained according to the present invention is effective for immunization of animals and humans against the virus disease. The vaccine is avirulent, scarcely subject to induction of unfavorable factors Cited by: This method is also used for the cultivation of some bacteria like Chlamydiae and Rickettsiae.

Figure 3:Routes of viral inoculation in embryonated egg. Advantages: Widely used method for the isolation of virus and growth. Ideal substrate for the viral growth and replication. Isolation and cultivation of many avian and few mammalian viruses.

Angiogenesis plays a very important role in tumor progression through the creation of new blood vessels. Therefore, angiogenesis inhibitors could contribute to cancer treatment.

Here, we show that a microbial metabolite, elaiophylin, exhibits potent antiangiogenic activity from in vitro and in vivo angiogenesis assays. Elaiophylin dramatically suppressed in vitro angiogenic Cited by: 6. Alice Miles Woodruff (also known as Alice Lincoln Miles) was an American virologist.

She developed a method for growing fowlpox outside of a live chicken alongside Ernest William Goodpasture. [1] [2] Her research greatly facilitated the rapid advancement in the study Alma mater: Mount Holyoke College, Yale University. Retinoic acid (RA) is an important signaling molecule in the development of the endoderm and an important molecule in protocols used to generate endodermal cell types from stem cells.

In this review, we describe the RA signaling pathway and its role in the patterning and specification of the extra embryonic endoderm and different endodermal by: 6. The disclosure describes a process for the production of an attenuated strain of measles virus effective for the production of an attenuated measles virus vaccine.

The starting material is an Edmonston strain of measles virus which has undergone a plurality of culture passages in various culture media.

A sample of the last culture passage is taken and a plurality of culture passages Cited by: 9. Nerve Growth Factor-Induced Angiogenesis: 2.

The Quail Chorioallantoic Membrane Assay Chapter (PDF Available) in Methods in Molecular Biology.“The susceptibility of the chorio-allantoic membrane of chick embryos to infection with the fowl-pox virus”, American journal of pathology, vii (), – 22; reprinted in Hahon, op.

cit. (ref. 25), – Cited by: The history of virology – the scientific study of viruses and the infections they cause – began in the closing years of the 19th century. Although Louis Pasteur and Edward Jenner developed the first vaccines to protect against viral infections, they did not know that viruses existed.

The first evidence of the existence of viruses came from experiments with filters that had pores small. ^ Steinhardt, E; Israeli, C; and Lambert, R.A. () "Studies on the cultivation of the virus of vaccinia" J.

Inf Dis. 13, – ^ Maitland HB, Magrath DI (September ). "The growth in vitro of vaccinia virus in chick embryo chorio-allantoic membrane, minced embryo and cell suspensions". The Journal of Hygiene 55 (3): – Author Summary Poxviruses are large DNA viruses that cause diseases in humans and other animals.

To initiate infection, the core of the large, membrane-enveloped particle must penetrate into the cytoplasm where replication occurs. For most enveloped viruses only one or two proteins are needed for attachment and penetration. However, at least sixteen poxvirus proteins are. These results confirm our earlier findings with chick embryos and growing chicks [12,13].

Moreover, our previous studies indicated that neither Cu nanoparticles nor silver or gold nanoparticles interfere with the growth and development of embryos when used at levels up to 50 ppm [14,15,16].Cited by: With Morris F.

Shaffer. Quantitative studies on the infectivity of the virus of herpes simplex for the chorio-allantoic membrane of the chick embryo, together with observations on the inactivation of the virus by its specific antiserum. Immunol. With H. The chorio-allantoic membrane in reptiles and birds contacts the surface membranes of the shell (fig.

E). In the higher mammals an allantoic diverticulum also is formed. In this group of vertebrates, the allantois not only serves the function of external respiration but also is the main instrument in nutrition.The murine allantois will become the umbilical artery and vein of the chorioallantoic placenta.

In previous studies, growth and differentiation of the allantois had been elucidated in whole embryos.The chick embryo is as ancient a source of knowledge on animal development as the very beginning of embryology.

Already, at the time of Caspar Friedrich Wolff, contemplating the strikingly beautiful scenario of the germ deploying on the yellow background of the yolk inspired and supported the tenants of epigenesis at the expense of the preformation by: